Proteomics techniques for cystic fibrosis research
نویسندگان
چکیده
منابع مشابه
Airway clearance techniques in cystic fibrosis.
Whilst the trials quoted in the Cochrane reviews [5–7] provide various degrees of quality of evidence, the key information that is missing from these studies is an understanding of the physiological principles by which these different techniques work. Physiologically, the techniques need to do the following. 1) Increase absolute peak expiratory flow (PEF) to move secretions towards the orophary...
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When primary human airway epithelial (hAE) cells are grown in vitro on porous supports at an air-liquid interface (ALI), they recapitulate in vivo morphology and key physiologic processes. These cultures are useful for studying respiratory tract biology and diseases and for testing new cystic fibrosis (CF) therapies. This chapter gives protocols enabling creation of well-differentiated primary ...
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Immortalization of human bronchial epithelial (hBE) cells often entails loss of differentiation. Bmi-1 is a protooncogene that maintains stem cells, and its expression creates cell lines that recapitulate normal cell structure and function. We introduced Bmi-1 and the catalytic subunit of telomerase (hTERT) into three non-cystic fibrosis (CF) and three DeltaF508 homozygous CF primary bronchial ...
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The systematic analysis of biological processes requires an understanding of the quantitative expression patterns of proteins, their interacting partners and their subcellular localization. This information was formerly difficult to accrue as the relative quantification of proteins relied on antibody-based methods and other approaches with low throughput. The advent of soft ionization technique...
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Cystic fibrosis (CF) is an inherited disease that primarily affects the lungs and the digestive system, however, it also affects a number of other organs and systems. More than 90% of mortality of CF patients is due to lung complications. Healthy lungs are important for a long life for people with CF, We will discuss two important topics for maintaining respiratory health. Chronic use of drug...
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ژورنال
عنوان ژورنال: Journal of Cystic Fibrosis
سال: 2004
ISSN: 1569-1993
DOI: 10.1016/j.jcf.2004.05.019